Figure 3.

(A) Schematic and photograph of imaging area on the hind limb after skin removal. (B, C) Intensity and DOP cross-section of the hind leg prior to GNBP₁₃₉₄ injection. (D, E) Intensity and DOP cross-section of the hind leg after to GNBP₁₃₉₄ injection. Lymphatic vessels (yellow) can be seen in (E). Longitudinal views of (F) intensity, (G) DOP, and (H) spectral contrast of the left lymphatic vessel. Note the negatively contrasting spectral shadow below most of the length of the positively contrasting lymphatic vessel. (I) Spectral contrast (SC) masked using DOP and overlaid onto the intensity image. (J) Enface intensity masked by angiography and (K) enface view of the tissue surface with masked spectral contrast. The green arrow identifies the saphenous vein. White arrows show low DOP the signal with the corresponding angiography signal, identifying lymphatic vessels. Unless otherwise noted, the horizontal scale bar is 1 mm and the vertical bar is 500 μm. The intensity is given on the dB scale. SC color scale: −1 to 1. DOP: 0.4 to 1.