Table 2. Antimycobacterial activity screening of five plant secondary metabolites against 30 M. abscessus isolates.

Isolates	Organism	Colony morphology	MIC value of clarithromycin (µg/mL)							Antimycobacterial screening against M. abscessus isolates
			Day 3		Day 5		Day 14		DST interpretation	MIC value of pure compounds (µg/mL)
			MIC	Phenotype	MIC	Phenotype	MIC	Phenotype		RL008	RL009	RL011	RL012	RL013
80097	M. abscessus subsp. abscessus	Mixed	0.25	Susceptible	8	Resistant	16	Resistant	Inducible	128	16	64	64	128
80167	M. abscessus subsp. massiliense	Rough	≥16	Resistant	≥16	Resistant	≥16	Resistant	Acquired	128	8	64	64	128
80225	M. abscessus subsp. abscessus	Rough	0.25	Susceptible	4	Intermediate	≥16	Resistant	Inducible	<1	<1	<1	<1	<1
80448	M. abscessus subsp. massiliense	Rough	0.12	Susceptible	0.25	Susceptible	2	Susceptible	Susceptible	128	128	128	128	128
80524	M. abscessus subsp. massiliense	Mixed	0.5	Susceptible	2	Susceptible	2	Susceptible	Susceptible	128	128	128	128	128
80700	M. abscessus subsp. massiliense	Rough	>16	Resistant	>16	Resistant	>16	Resistant	Acquired	>128	128	64	>128	128
80824	M. abscessus subsp. massiliense	Mixed	0.25	Susceptible	0.5	Susceptible	1	Susceptible	Susceptible	128	16	32	128	128
80838	M. abscessus subsp. abscessus	Mixed	1	Susceptible	16	Resistant	16	Resistant	Inducible	128	128	128	128	>128
80866	M. abscessus subsp. massiliense	Smooth	0.12	Susceptible	0.12	Susceptible	>16	Resistant	Inducible	128	128	128	128	128
80901	M. abscessus subsp. abscessus	Rough	1	Susceptible	8	Resistant	16	Resistant	Inducible	>128	128	128	128	>128
80988	M. abscessus subsp. abscessus	Rough	0.12	Susceptible	0.25	Susceptible	16	Resistant	Inducible	128	>128	64	128	>128
81103	M. abscessus subsp. abscessus	Smooth	2	Susceptible	2	Susceptible	16	Resistant	Inducible	128	128	64	128	128
81350	M. abscessus subsp. massiliense	Rough	≤0.06	Susceptible	0.12	Susceptible	0.12	Susceptible	Susceptible	>128	128	128	128	128
81422	M. abscessus subsp. abscessus	Rough	0.5	Susceptible	1	Susceptible	≥16	Resistant	Inducible	>128	128	128	128	>128
81463	M. abscessus subsp. massiliense	Smooth	≤0.06	Susceptible	0.12	Susceptible	0.25	Susceptible	Susceptible	128	128	64	128	128
81499	M. abscessus subsp. massiliense	Rough	16	Resistant	16	Resistant	16	Resistant	Acquired	128	128	64	128	128
81618	M. abscessus subsp. abscessus	Mixed	16	Resistant	16	Resistant	16	Resistant	Acquired	128	16	32	128	128
81652	M. abscessus subsp. massiliense	Mixed	0.12	Susceptible	0.25	Susceptible	1	Susceptible	Susceptible	128	8	128	64	128
81702	M. abscessus subsp. massiliense	Rough	>16	Resistant	>16	Resistant	>16	Resistant	Acquired	>128	128	64	>128	128
81838	M. abscessus subsp. massiliense	Rough	16	Resistant	16	Resistant	≥16	Resistant	Acquired	128	128	128	128	128
82119	M. abscessus subsp. abscessus	Smooth	2	Susceptible	16	Resistant	16	Resistant	Inducible	128	16	64	64	128
82154	M. abscessus subsp. abscessus	Smooth	0.5	Susceptible	8	Resistant	16	Resistant	Inducible	128	32	64	64	128
82593	M. abscessus subsp. massiliense	Rough	≤0.06	Susceptible	0.12	Susceptible	0.5	Susceptible	Susceptible	>128	128	128	>128	>128
82895	M. abscessus subsp. massiliense	Rough	0.25	Susceptible	0.5	Susceptible	2	Susceptible	Susceptible	128	>128	32	128	>128
82998	M. abscessus subsp. abscessus	Smooth	16	Resistant	16	Resistant	16	Resistant	Acquired	128	128	128	128	128
83305	M. abscessus subsp. abscessus	Smooth	16	Resistant	16	Resistant	16	Resistant	Acquired	>128	128	128	128	128
83310	M. abscessus subsp. abscessus	Rough	8	Resistant	8	Resistant	≥16	Resistant	Acquired	128	>128	128	>128	>128
83347	M. abscessus subsp. massiliense	Mixed	0.25	Susceptible	0.5	Susceptible	0.5	Susceptible	Susceptible	128	128	128	128	>128
90919	M. abscessus subsp. massiliense	Mixed	0.12	Susceptible	0.12	Susceptible	0.5	Susceptible	Susceptible	128	128	128	128	>128
826492	M. abscessus subsp. massiliense	Mixed	0.12	Susceptible	0.25	Susceptible	16	Resistant	Inducible	128	16	64	64	128

Note:

MIC, minimum inhibitory concentration; DST, drug susceptibility testing; Acquired, acquired resistance; Inducible, inducible resistance.