Figure 2.

KDM6B binds to several WNT pathway gene promoters upon Salmonella infection: [A] Schematic representation of procedure employed for EpiTectChIP qPCR array. [B] Analysis of KDM6B binding at promoter of Cox2 gene in HCT-8 cells 4h p.i. of SL using ChIP. [C] Volcano plot showing KDM6B fold enrichment at several WNT pathway gene promoter post 4 h SL infection versus control in HCT-8 Cells using EpiTect qPCR array (Red dots are indicative of genes displaying p-value <.05; data is representative of two independent experiments). [D] Expression analysis of identified KDM6B target genes by above ChIP qPCR array at 4 h post SL infection in HCT-8 versus control cells. [E] Expression analysis of identified KDM6B target genes expression upon SL infection in the presence of KDM6B demethylase inhibitor GSKJ4 (30 µM). [F] Fold change in PPARδ expression at RNA level in RAW264.7 macrophages 4h and 18h p.i. w.r.t to control.(HPRT was used for normalization). Statistical significance was analyzed using unpaired t-test. (‘***’ p-value <.001;’**’ p-value <.01; ‘*’ p- value <.05)