Figure 5.

KDM6B demethylase activity is necessary for intracellular survival of Salmonella in murine MLNs: [A] Immunoblots showing increased expression of KDM6B mice in mesentric lymph nodes, Peyer’s patches and intestinal crypts in streptomycin pretreated mice model of SL 48h p.i. [B] Graphical representation of densitometry data for the above mentioned blots respectively (n = 6 mice per group). [C] Immunoblot showing KDM6B expression in colon at 48h p.i. of SL in C57BL/6 mice; Actin was used as loading control. [D] Fold change in KDM6B and PPARδ expression at RNA level in MLNs at 48h post SL infection in streptomycin pretreated mice colitis model using qPCR (n = 3 mice per group; HPRT was used for normalization). [E] Schematic representation of KDM6B demthylase inhibitor GSKJ4 mice Salmonella colitis model. [F-G] SL Colony forming units (CFU) in MLN, Peyer’s patches, colon and spleen in vehicle (DMSO) and GSKJ4 treated mice 48h p.i. (results are cumulative from 3 independent experiments). CFU of SL in RAW264.7 macrophages when treated with GSKJ4 (i), and upon knocking down of KDM6B by siRNA (KDM6B KD) (h) at 4h and 18h p.i. checked using GPAs. [J] KDM6B expression at RNA level in mouse KDM6B siRNA transfected RAW264.7 cells. Statistical significance was analyzed using unpaired t test and Mann-Whitney U Test. (’***’- p-value <.001; ‘**’- p-value <.01, ‘*’p-value <.05, ns-not significant)