Table 2.
List of primer sequence used
| Sr.No. | Gene name | Primer Sequence 5ʹ-3’ |
|---|---|---|
| Human Primer Sequence: | ||
| 1. | KDM6B Forward | GCACTACTGGGAGACCATCA |
| 2. | KDM6B Reverse | ACCAGGAACCCGTCAAGTAG |
| 3. | HPRT Forward | GCTATAAATTCTTTGCTGACCTGCTG |
| 4. | HPRT Reverse | AATTAACTTTTATGTCCCCTGTTGACTGG |
| 5. | KDM6A Forward | TACAGGCTCAGTTGTGTAACCT |
| 6. | KDM6A Reverse | CTGCGGGAATTGGTAGGCTC |
| 7. | NSD3 Forward | GTGTTAAGTTTCAGGTTGGCG |
| 8. | NSD3 Reverse | CTGGACATGATATTCTCGGGC |
| 9. | APC Forward | CATGATGCTGAGCGGCAGA |
| 10. | APC Reverse | GCTGTTTCATGGTCCATTCGTG |
| 11. | DAAM1 Forward | TCACCCAGAAATCACGTATCG |
| 12. | DAAM1 Reverse | TCTGTGTTTGTCTGTGAGGTC |
| 13. | FZD4 Forward | TACCTCACAAAACCCCCATCC |
| 14. | FZD4 Reverse | GGCTGTATAAGCCAGCATCAT |
| 15. | PPARδ Forward | GTCACACAACGCTATCCGTTT |
| 16. | PPARδ Reverse | AGGCATTGTAGATGTGCTTGG |
| 17. | WNT3 Forward | GGAGAAGCGGAAGGAAAAATG |
| 18. | WNT3 Reverse | GCACGTCGTAGATGCGAATACA |
| 19. | EP300 Forward | GACCAGACTACAGAAGCAGAAC |
| 20. | EP300 Reverse | CACGGATCATACTTGGGTCAG |
| 21. | CSNK1D Forward | CATCCCCTATCGTGAGAACAAG |
| 22. | CSNK1D Reverse | AGCCCAGGTTGAAGTACATTAG |
| Mouse Primer Sequence: | ||
| 1. | HPRT Forward | GCTGGTGAAAAGGACCTCT |
| 2. | HPRT Reverse | CACAGGACTAGAACACCTGC |
| 3. | KDM6B Forward | TCGCTAAATACGCACAGTACC |
| 4. | KDM6B Reverse | GGTTCCTGTAGTGCTGTCTG |
| 5. | PPARδ Forward | CGGGCTCTAGAATTCCATCTG |
| 6. | PPARδ Reverse | AGGTCTCACTCTCCGTCTTC |
| 7. | IL1β Forward | CAGGATGAGGACATGAGCACC |
| 8. | IL1β Reverse | CTCTGCAGACTCAAACTCCAC |
| 9. | IFNγ Forward | TCTTGGCTTTGCAGCTCTTC |
| 10. | IFNγ Reverse | TGTTGCTGATGGCCTGATTG |
| 11. | ARG Forward | GGAATCTGCATGGGCAACCTGTGT |
| 12. | ARG Reverse | AGGGTCTACGTCTCGCAAGCCA |
| 13. | YM1 Forward | TCTGGTGAAGGAAATGCGTAAA |
| 14. | YM1 Reverse | GCAGCCTTGGAATGTCTTTCTC |
| 15. | IL10 Forward | TGCACTACCAAAGCCACAAG |
| 16. | IL10 Reverse | TCAGTAAGAGCAGGCAGCAT |
| 17. | IL4RA Forward | AGAGAATGTTAGTGTCAGTGTGG |
| 18. | IL4RA Reverse | ACATGCTCAGGTCCTCTTTG |
| 19. | ADRP Forward | AACAAAAGAGCCAGGAGACC |
| 20. | ADRP Reverse | CCACGAGACATAGAGCTTATCC |
| 21. | CPT1A Forward | ATCACCCCAACCCATATTCAG |
| 22. | CPT1A Reverse | TGCGGGAAGTATTGAAGAGTC |
| 23. | UCP2 Forward | TCCTGAAAGCCAACCTCATG |
| 24. | UCP2 Reverse | GGCAGAGTTCATGTATCTCGTC |
| 25. | ACADM Forward | CCGGAACACTTACTATGCCTC |
| 26. | ACADM Reverse | TGTTGAATCCATAGCCTCCG |
| ChIP qPCR Primer Sequence | ||
| 27. | COX2_P Forward | AAGGGGAGAGGAGGGAAAAATTTGTG |
| 28. | COX2_P Reverse | GAGGCGCTGCTGAGGAGTTCCTG |
| 29. | GAPDH_P Forward | CTGCAGTACTGTGGGGAGGT |
| 30. | GAPDH_P Reverse | CAAAGGCGGAGTTACCAGAG |
| 31. | PPARδ _P Forward | TTTGACCAGCCCTAACTTCTC |
| 32. | PPARδ_P Reverse | CCCCACAACTAAGCACTCG |
| Primers for generation of SteE Knockout | ||
| 33. | SteEKO1 | GATCATACTTGTGGTTTTCCTTAGGAGGTAACATGTTTACAGTGTAGGCTGGAGCTGCTT |
| 34. | SteEKO2 | CTATTCGGCGCAGCTATTTATAACGCTTTGTTTTATTCAGTCATATGAATATCCTCCTTAG |
| 35. | SteE1 | AATTTAAGCTTAATGTTTACAATTAATAGTACTAACAGG |
| 36. | SteE2 | ATATGAATTCTCTTCATCCGGGAAAACC |
| 37. | Cat1 | TTATACGCAAGGCGACAAGG |
| 38. | Cat2 | GATCTTCCGTCACAGGTAGG |