Figure 2.

Transduction rate of lentiviral plasmid in microglia. Neonatal rat microglia were plated in six-well plates with complete medium 24 h prior infection. Next day, microglia were infected with IRE1 overexpressed lentivirus at different multiplicity of infection (MOI) value (10–80) and incubated for 12 h. Microglia were gathered 72 h after transfection, and transduction efficiency was measured with an inverted fluorescence microscopy (Olympus, Tokyo, Japan). Green signal represents infected cells. Transduction rate was approximately 90% at an MOI of 80. W: observation under white light; G: observation under green light.