Figure 1.

Circulating γδ T are more differentiated than T CD8 lymphocytes in most healthy individual’s blood. a, pseudotimed trajectory of PBMC-derived γδ T cells. Cell color key: pseudotime-ordered milestones. b, mean of gene expression per milestone. Genes shown blue and red: naïve (Tn) and effector (Teff) signatures, respectively. c, Single cell scores for Tn and Teff signatures across the trajectory. d, dendrogram of milestones (centroids, same color key as a) overlaid to the pseudotimed trajectory showing all γδ T cells (gray dots), marker genes, trajectory (dark line), and differentiation direction (lines). e-h, same as a-d for T CD8 cells extracted from the same PBMC. i, rates of γδ T and T CD8 cells at each differentiation stage. Abbreviations: Tn: naïve T, Tcm: central memory T, Teff: T effector, Tcm-mig: migration-prone central memory T, Tem: effector memory T, Tem-act: activated effector memory T, Tem-cyto: cytotoxic effector memory T, Temra: effector memory CD45RA T, Tscm: stem-like central memory T, Tpex: progenitor exhausted-like T