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. 2021 Oct 25;56(20):2886–2901.e6. doi: 10.1016/j.devcel.2021.09.021
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

GFP-Booster Alexa Fluor 488 Chromotek Cat# gb2AF488-10

Bacterial and virus strains

Sf9 cells Expression systems Cat#94-001F
Subcloning Efficiency DH5α Competent Cells Invitrogen Cat#18265017

Chemicals, peptides, and recombinant proteins

Cytochalasin-D Sigma-Aldrich Cat# C8273
1,6 Hexanediol (1,6-HD) Sigma-Aldrich Cat# 240117
RNase A Roche Cat# RNASEA-RO
cOmplete Protease Inhibitor Cocktail, EDTA-free Roche Cat#5056489001
1X Dulbecco’s Phosphate Buffered Saline (PBS) solution without MgCl2 Sigma-Aldrich Cat# D8537
Potassium chloride Merck Cat#104935
DTT Fermentas Life
Sciences
Cat#R0862
Benzonase Produced in-house N/A
Tris Carl Roth Cat# 5429
Amylose resin NEB Cat# E8021S
EDTA Roche Cat# 105063
Pipes Applichem Cat# A1079
monoGFP Produced in-house N/A
Activation Buffer (AB) York-Andersen et al., 2015 N/A
Schinder’s Drosophila medium Gibco Cat# 21720024
‘Wash Buffer A’ for Stellaris RNA FISH LG Biosearch Technologies Cat# SMF-WA1-60
‘Hybridisation Buffer’ for Stellaris RNA FISH LG Biosearch Technologies Cat# SMF-HB1-10
‘Probe Hybridisation Buffer’ for HCR V3.0 Molecular Instruments Bundled with custom probe set
‘Probe Wash Buffer’ for HCR V3.0 Molecular Instruments Bundled with custom probe set
‘Amplification Buffer’ for HCR V3.0 Molecular Instruments Bundled with custom probe set
SlowFade Diamond Antifade Mountant with DAPI Thermofisher Scientific S36934
Protease inhibitor cocktail Roche Cat# CO-RO
Amylose Resin New England Biolabs Cat# E8021S
PreScission Protease GST 3C GE Life Sciences Cat# GE27-0843-01
Polyethene Glycol – 2000 Merck Cat#817018
Recombinant GFP-Me31B protein This paper N/A
Recombinant GFP-Me31BΔN-ΔC protein This paper N/A

Critical commercial assays

Size exclusion chromatography using a HiLoad 16/600 Superdex 200 pg GE Life Sciences Cat# GE28-9893-35
Amicon Ultra-0.5 Centrifugal Filter Unit Millipore Cat# UFC5030

Deposited data

Raw set of disordered regions from the Drosophila proteome This paper https://github.com/holehouse-lab/supportingdata/tree/master/2021/sankaranarayanan_me31b_2021

Experimental models: Organisms/strains

D. melanogaster: y[1] w[]; P{w[+mC]=PTT-GB}me31B[CB05282]
(Me31B::GFP)
Bloomington Drosophila Stock Centre (Buszczak et al., 2007) BDSC: 51530
FlyBase: FBst0051530
D. melanogaster: y,w, bcd-(ms2)6 (18), bcd-(ms2)6 (4); hsp83-MCP-RFP(4a) Weil et al., 2006 N/A
D. melanogaster: GFP::Tral Drosophila Genomics Resource Centre (Morin et al., 2001) DGRC: 110584
Flytrap:G00089;DGRC:110584;RRID:DGGR_110584
D. melanogaster: y[1]; P{y[+mDint2] w[BR.E.BR]=SUPor-P} tral[KG08052] ry[506] / TM3, Sb[1] Ser[1]
(tral1)
Bloomington Drosophila Stock Centre (Wilhelm et al., 2005) BDSC: 14933FlyBase:
FBgn0041775
D. melanogaster: w[1118]; Df(3L)ED4483, P{w[+mW.Scer\FRT.hs3]=3’.RS5+3.3’}ED4483/ TM6C, cu[1] Sb[1] Bloomington Drosophila Stock Centre (Wilhelm et al., 2005) BDSC: 8070FlyBase:
FBab0035731

Oligonucleotides

Custom Stellaris FISH Probes for the 3’UTR of bcd RNA Stellaris See Table S2 (supplemental information).
Molecular Instruments Custom HCR Probe for hb RNA Molecular Instruments DNA Custom kit
Drosophila melanogasterlot number: 2690/B795GenBank:
NM_169234.2, Alexa647, v3.0 kits

Recombinant DNA

Recombinant GFP-Me31B plasmid This paper N/A
Recombinant GFP-Me31BΔN-ΔC plasmid This paper N/A

Software and algorithms

ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/
ImageJ Plugin Trackmate Tinevez et al., 2017 https://imagej.net/plugins/trackmate/
Rstudio/ R software RStudio Team 2021 http://www.rstudio.com/.
HullRad Fleming and Fleming, 2018 N/A
ABSINTH implicit solvent model Vitalis and Pappu, 2009 N/A
CAMPARI Monte Carlo simulation (v3.0) http://campari.sourceforge.net/V3/index.html N/A
SWISS-MODEL Waterhouse et al., 2018 N/A
SOURSOP https://soursop.readthedocs.io/ N/A
MDTraj McGibbon et al., 2015 N/A
DSSP Algorithm Kabsch and Sander, 1983 N/A
Protfasta https://protfasta.readthedocs.io/ N/A
Metapredict Emenecker et al., 2021 N/A
LocalCIDER Holehouse et al., 2017 N/A
PIMMS simulation engine Martin et al., 2020 N/A

Other

Iberian recipe fly food Produced in-house N/A
Oil 10 S (95 Series Halocarbon Oil) VWR Chemicals N/A
Olympus FV3000 Confocal Laser Scanning Microscope Olympus FV3000
DeltaVision Core widefield microscope Applied Precision, LLC DeltaVision Core
Custom built dual-trap optical tweezer instrument Jahnel et al., 2011 N/A