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. Author manuscript; available in PMC: 2022 Jun 25.
Published in final edited form as: Biochem J. 2021 Jun 25;478(12):2385–2397. doi: 10.1042/BCJ20210158

Fig 2).

Fig 2)

Precipitation studies of purified PlyCB and GAC reveal direct interaction of PlyCB with GAC. A) The PlyCB concentration is kept constant whilst the GAC concentration is varied. Visible precipitate forms at the higher concentrations. B) The precipitate level is measured spectrophotometrically at 340 nm and compared to the mutant PlyCBR66E, which does not bind the GAC. C) Coomassie stained and D) densitometry analysis of PlyCB protein from the supernatant fraction and aggregates (pellets). E, F) The same dose dependency is observed when the PlyCB concentration is varied. Arrowhead depicts PlyCB protein at 8 kDa.