The PlyC-mediated lysis of sacculi purified from S. mutans strains. The lysis was monitored after 10, 20, 30, 40, 50 and 60 min as a decrease in OD600. Results are presented as a fold change in OD600 of the sacculi incubated with PlyC vs. the sacculi incubated without PlyC. Data points and error bars represent mean values of four biological replicates and standard deviation, respectively. P-values were determined by two-way ANOVA with Dunnett’s multiple comparisons test.