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Schematic of the SPT5 protein. (A) A schematic of the SPT5 protein with the positions of the acidic domain, the four KOW domains that have homology to the E. coli NusG protein, and two domains, CTR1 and CTR2, each of which has multiple amino acid repeats, is shown. (B) A variety of contructs with mutations in different domains of the SPT5 protein are shown. These mutants were analyzed for their function using both in vivo interaction and in vitro transcription assays.
