Fig. 1.

a Increased activation of the sympathetic nervous system due to stress-related emotional or physical events, increases catecholamines (epinephrine, norepinephrine, dopamine), and this increase stimulates adrenergic receptors. Specifically, transmissions in signal transduction pathways triggered by the agonist response of β-ARs act as essential regulators of heart rate, systolic and diastolic function, and myocardial metabolism and thus systolic dysfunction may more readily develop in the left ventricle of the heart under stress.(Created in BioRender.com). b This protocol describes how to carry out native barcoding of genomic DNA using the Native Barcoding Expansion 1–12 (EXP-NBD104), in conjunction with the Ligation Sequencing Kit (SQK-LSK109)