Fig. 4. EPS-8 mediates mitochondrial homeostasis through integrin.

(A) Fluorescent micrographs of day 1 adult hsp-6p::GFP animals grown on ev control or eps-8 RNAi mixed in a 1:1 ratio with empty vector control, ina-1, or pat-3 RNAi from hatch. All images are contrast-matched. (B) Quantification of hsp-6p::GFP in day 1 adult animals grown on empty vector control (gray) or eps-8 RNAi (red) mixed in a 1:1 ratio with empty vector control, ina-1, or pat-3 RNAi from hatch. Lines represent median and interquartile range, with each dot representing a single animal. n = 156 to 358 per strain. Data are representative of three independent trials. ***P < 0.001; ns, P > 0.10 using nonparametric Mann-Whitney testing. (C and D) Representative fluorescent images of day 1 adult animals expressing a mitochondria-targeted GFP from a muscle-specific promoter, myo-3p (C) or a hypodermal-specific promoter, col-19p (D). Animals were grown on empty vector control or eps-8 RNAi mixed in a 1:1 ratio with empty vector control, ina-1, or pat-3 RNAi from hatch and imaged directly on glass slides as described in Materials and Methods. Muscle images were captured on a standard wide-field Zeiss AxioObserver Z1. Hypodermal images were captured on an LSM900 Airyscan microscope. Scale bars, 10 μm. (E and F) Quantification of (C) and (D) using MitoMAPR. For muscle (E), quantification was performed on two to three muscle cells for 6 to 10 animals per sample on single slice images. For hypodermis (F), quantification was performed on single image max projections for 6 to 10 animals per sample. *P < 0.05 and ***P < 0.001; ns, P > 0.10 using nonparametric Mann-Whitney testing.