TABLE 2.
Inhibition of ergosterol biosynthesis in C. albicans isolates by fluconazole
| Classification of isolatesa | Mean ergosterol contentb of cells grown with fluconazole at a concn (μg/ml) of:
|
||||
|---|---|---|---|---|---|
| 0 | 1 | 4 | 16 | 64 | |
| Susceptible (n = 18) | 1.4 ± 0.07 | 0.4 ± 0.1 (72)c | 0.23 ± 0.06 (84)c | 0.07 ± 0.03 (95)c | 0 ± 0 (100)c |
| SDD (n = 10) | 1.6 ± 0.14 | 1.0 ± 0.11 (38) | 0.69 ± 0.17 (57) | 0.43 ± 0.13 (73)c | 0.005 ± 0.003 (99)c |
| Resistant (n = 10) | 1.6 ± 0.13 | 1.2 ± 0.09 (25) | 1.0 ± 0.13 (38) | 0.8 ± 0.13 (53) | 0.2 ± 0.13 (84)c |
a
By the NCCLS broth microdilution assay.
b
Expressed as a percentage of the wet weight of the cell ± the standard error of the mean (followed in parentheses by the percent reduction in the mean cellular ergosterol content compared with that of control cells grown without fluconazole).
c
Significant reduction compared with controls (P < 0.05).