| Strain, strain background (Danio rerio) |
AB |
ZIRC |
RRID: ZL1ZFIN ID: ZDB-GENO-960809–7 |
|
| Strain, strain background (Danio rerio) |
Tübingen |
ZIRC |
RRID: ZIRC_ZL57ZFIN ID: ZDBGENO-990623–3 |
|
| Genetic reagent (Danio rerio) |
lhfpl5bvo35/vo35
|
Erickson et al., 2019
|
RRID:ZIRC_ZL13656.05ZFIN ID: ZDB-GENO-200824–4 |
|
| Genetic reagent (Danio rerio) |
TgBAC(neurod1:EGFP) |
Obholzer et al., 2008
|
ZFIN ID: ZDB-ALT-080701–1 |
|
| Genetic reagent (Danio rerio) |
Tg(myo6b:actb1-EGFP) |
Kindt et al., 2012
|
ZFIN ID: ZDB-TGCONSTRCT-120926–1 |
|
| Genetic reagent (Danio rerio) |
Tg(mpeg1:YFP) |
Roca and Ramakrishnan, 2013
|
ZFIN ID: ZDB-ALT-130130–3 |
|
| Sequence-based reagent |
lhfpl5b_ F |
Erickson et al., 2019
|
PCR primer |
GCGTCATGTGGGCAGTTTTC; Made by IDT |
| Sequence-based reagent |
lhfpl5b_R |
Erickson et al., 2019
|
PCR primer |
TAGACACTAGCGGCGTTGC; Made by IDT |
| Antibody |
(Ribbon label: Mouse monoclonal anti-Ribeye b IgG2a) |
Sheets et al., 2011
|
N/A |
(1:10,000) |
| Antibody |
(Ribbon label: Mouse monoclonal anti-panCtBP IgG2a) |
Santa Cruz |
Cat. No. sc-55502 |
(1:1000) |
| Antibody |
(PSD label: Mouse monoclonal anti-panMAGUK IgG1) |
NeuroMab |
K28/86, #75–029 |
(1:500) |
| Antibody |
(Chicken polyclonal anti-GFP) |
Aves Labs |
Cat. No. GFP-1020 |
(1:500) |
| Antibody |
(Hair cell label: Rabbit polyclonal anti-Parvalbumin) |
Thermo Fisher |
Cat. No. PA1-933 |
(1:500) |
| Antibody |
(Hair cell label: Rabbit polyclonal anti-Parvalbumin) |
Abcam |
Cat. No. ab11427 |
(1:2000) |
| Antibody |
(Hair cell label: Mouse anti-Otoferlin IgG2a) |
Developmental Studies Hybridoma Bank |
HCS-1 |
(1:500) |
| Antibody |
(Goat anti-Rabbit IgG Secondary Antibody, Pacific Blue) |
Thermo Fisher |
Cat. No. P-10994 |
(1:400) |
| Antibody |
(Goat anti- Mouse IgG1 Antibody, Alexa Fluor 488) |
Thermo Fisher |
Cat. No. A-21121 |
(1:1000) |
| Antibody |
(Goat anti-Chicken IgY Antibody, Alexa Fluor 488) |
Thermo Fisher |
Cat. No. A-11039 |
(1:1000) |
| Antibody |
(Goat anti-Rabbit IgG Antibody, Dylight 549) |
Vector Laboratories |
Cat. No. DI-1549–1.5 |
(1:1000) |
| Antibody |
(Goat anti-Rabbit IgG Antibody, Alexa Fluor 555) |
Thermo Fisher |
Cat. No. A27039
|
(1:1000) |
| Antibody |
(Goat anti- Mouse IgG2a Antibody, Alexa Fluor 647) |
Thermo Fisher |
Cat. No. A-21241 |
(1:1000) |
| Peptide, recombinant protein |
MluCI |
New England Biolabs |
Cat. No. R0538 |
|
| Chemical compound, drug |
DL-TBOA |
Tocris |
Cat. No.1223 |
|
| Chemical compound, drug |
Copper(II) sulfate (CuSO4) |
Millipore Sigma |
Cat. No. 451,657 |
|
| Chemical compound, drug |
2.5 % Glutaraldehyde in 0.1 M Sodium Cacodylate Buffer, pH 7.4: SEM |
Electron Microscopy Sciences |
Cat. No. 15,960 |
|
| Chemical compound, drug |
Paraformaldehyde; IHC |
Millipore Sigma |
Cat. No. 158,127 |
|
| Commercial assay or kit |
Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 555 dye |
Thermo Fisher |
Cat. No. C10338
|
|
| Software, algorithm |
FIJI is just ImageJ |
NIH |
https://imagej.net/software/fiji/
|
|
| Software, algorithm |
Volocity |
Quorum Technologies |
https://quorumtechnologies.com/index.php/component/content/category/31-volocity-software
|
|
| Software, algorithm |
Prism (v9) |
Graphpad Software |
https://www.graphpad.com/
|
|
| Software, algorithm |
Adobe Illustrator |
Adobe |
https://www.adobe.com/
|
|
| Other |
FM1-43X; fixable analog of FM 1–43 |
Thermo Fisher |
Cat. No. F35355
|
3 µM for 20 seconds |
| Other |
DAPI nuclear stain |
Thermo Fisher |
Cat. No. Cat. No. F35355
|
5 mg/ml stock; diluted (1:2000) |
