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. 2021 Oct 8;10:e57462. doi: 10.7554/eLife.57462

Figure 6. TAZ represses glucocorticoid receptor (GR) transactivation of gluconeogenic genes in mouse liver.

(A, G) Endogenous TAZ was immunoprecipitated from liver nuclear extracts prepared from ad libitum-fed C57BL/6J mice (A) or mice treated with RU486 or vehicle (G), and the amounts of GR in the immunoprecipitates were measured by immunoblotting. C57BL/6J mice were administered AdshTAZ or AdshCon, as in Figure 2 (B, C, H) or AdTAZ or AdGFP, as in Figure 3 (D–F and K–N). (I, J) L-TAZ KO and control mice. (H–N) Mice were treated with RU486 (RU) or vehicle (V), as indicated. The relative enrichment of GR (B, D, N), acetylated-histone 4 (Ac-H4) (C, E, N), and Pol II (F) in the liver extracts was assessed using chromatin immunoprecipitation (ChIP) assays. (H, I, K) Blood glucose concentration. (L) Hepatic gene expression. (J, M) Pyruvate tolerance testing (PTT). Data are means and SEMs; n = 5–8; for ChIP, the data are the results of triplicate or quadruplicate immunoprecipitations. Data were analyzed by two-way ANOVA (B–F, I, J, M, N) and unpaired Student’s t-test (H, K, L). *p<0.05, **p<0.01, ***p<0.001, #p<0.05, ##p<0.01, ###p<0.001; in (B–F, N), # denotes comparisons with IgG; in (I, J), # denotes comparisons with vehicle-treated controls of the same genotype; NS, not significant.

Figure 6.

Figure 6—figure supplement 1. TAZ does not bind to gluconeogenic gene promoters.

Figure 6—figure supplement 1.

8- to 12-week-old male C57BL/6J mice were administered adenoviruses expressing TAZ (flag-tagged) or GFP for 5 days. Chromatin immunoprecipitation (ChIP) assays were performed from liver extracts using indicated antibodies. Data are means and SEMs of 3–4 immunoprecipitates. Data were analyzed by two-way ANOVA; * p<0.05, **p<0.01, #p<0.05, ##p<0.01, ###p<0.001; # denotes comparisons with IgG.
Figure 6—figure supplement 2. Blood glucose concentrations and hepatic gene expression of mice administered AdTAZ and dexamethasone (Dex).

Figure 6—figure supplement 2.

8- to 12-week-old C57BL/6J J mice were administered adenoviruses expressing TAZ or GFP for 5 days. Mice were treated with Dex or vehicle. Blood glucose (A) and hepatic gene expression (B) were measured. Data were analyzed by one-way ANOVA; *p<0.05, **p<0.01, ***p<0.001.