| Strain, strain background(Xenopus tropicalis, females) |
Wild-typeadult females |
Nasco |
LM00823 |
|
| Strain, strain background(X. tropicalis, males) |
Wild-typeadult males |
Nasco |
LM00822 |
|
| Strain, strain background(X. laevis, females) |
Wild-typeadult females |
Nasco |
LM00531 |
|
| Strain, strain background(X. laevis, males) |
Wilt-typeadult males |
Nasco |
LM00715 |
|
| Genetic reagent(X. tropicalis) |
Xtr.Tg(WntREs: dEGFP)Vlemx
|
National Xenopus
Resource (NXR) Center,Woods Hole, MA |
RRID:NXR_1094
|
X. tropicalis Wnt/Bcat reporter line |
| Genetic reagent(X. laevis) |
Xla.Tg(WntREs: dEGFP)Vlemx, |
NXR |
RRID:NXR_0064
|
X. laevis Wnt/Bcat reporter line |
| Genetic reagent(X. laevis) |
Xla.Tg.(nkx2-5:GFP)Mohun
|
NXR |
RRID:NXR_0030
|
X. laevis Nkx2-5:GFP reporter line |
| Strain, strain background(Mus musculus) |
CD-1 |
Charles River Labs |
Strain Code022RRID:IMSR_CRL:022
|
WT mice |
| Genetic reagent(M. musculus) |
Shhtm1(EGFP/cre)Cjt
|
Jax Labs |
JAX: 005622RRID:IMSR_JAX:005622
|
Shh:GFP mice |
| Cell line(M. musculus) |
Tbx5OE-mESC line |
Steimle et al., 2018
|
Steimle et al., 2018
|
|
| Chemical compound, drug |
Doxycycline |
Sigma-Aldrich |
D9891 |
|
| Antibody |
(Rabbit polyclonal) anti-Aldh1a2 |
Abcam |
ab96060, RRID:AB_10679336
|
IF (1:500) |
| Antibody |
(MouseMonoclonal)Anti-Aldh1/2 |
Santa Cruz Biotechnology |
sc-166362, RRID:AB_2009458
|
IF (1:500) |
| Antibody |
(Mouse monoclonal) anti-Sox2 |
Abcam |
ab79351; RRID:AB_10710406
|
IF (1:1000) |
| Antibody |
(Rabbit polyclonal) anti-Nkx2-1 (H-190) |
Santa CruzBiotechnology |
sc-13040X; RRID:AB_793532
|
IF (1:500) |
| Antibody |
(Mouse monoclonal) anti-Fibronectin (4H2) |
Developmental Studies Hybridoma Bank |
DSHB #4H2; RRID:AB_2721949
|
IF (1:2000) |
| Antibody |
(Chicken polyclonal) anti-GFP |
Aves Labs |
GFP-1020; RRID:AB_10000240
|
IF (1:1000) |
| Antibody |
(Goat polyclonal)anti-Tbx5 |
Santa Cruz Biotechnology |
sc-17866, RRID:AB_2200827
|
IF (1:300)ChIP: 5 µg |
| Recombinant DNA reagent |
pI-SceI-d2EGFP plasmid |
Addgene |
Addgene_32674
|
For meganuclease transgenics |
| Recombinant DNA reagent |
pRL-TK(plasmid) |
Promega |
E2241 |
|
| Recombinant DNA reagent |
pGL4.23 luc2/miniP(plasmid) |
Promega |
E8411 |
|
| Recombinant DNA reagent |
pCS2+ GR-xTbx5 |
Addgene |
Addgene 117248
|
|
| Recombinant DNA reagent |
pCS2+ xTbx5 |
Addgene |
Addgene 117247
|
|
| Recombinant DNA reagent |
pCSf107mT-Gateway-3′myc |
Addgene |
Addgene 67617
|
|
| Recombinant DNA reagent |
pENTR223Human TBX5 |
Horizon Discovery |
OHS5894-202500411 |
|
| Commercial assay or kit |
Gateway LR Clonase II enzyme mix |
Thermo Fisher Scientific |
11791020 |
|
| Commercial assay or kit |
mMessage mMachine SP6 RNA synthesis kit |
Thermo Fisher Scientific |
AM1340 |
|
| Peptide, recombinant protein |
FGF8b |
R&D Systems |
423-F8-025 |
|
| Peptide, recombinant protein |
WNT2B |
R&D Systems |
3900-WN-025 |
|
| Commercial assay or kit |
TRIzol |
Thermo Fisher Scientific |
15596018 |
|
| Commercial assay or kit |
Direct-zolMiniprep plus kit |
Thermo Fisher Scientific |
R2070 |
|
| Commercial assay or kit |
SuperscriptVILO mastermix |
Thermo Fisher Scientific |
11755050 |
|
| Commercial assay or kit |
PowerUP2× SYBR Green MasterMix |
Thermo Fisher Scientific |
A25742 |
|
| Commercial assay or kit |
Firefly Luciferase 2.0 kit |
Biotium |
30085-1 |
|
| Commercial assay or kit |
Renilla Luciferase2.0 kit |
Biotium |
30082-1 |
|
| Chemical compound, drug |
DEAB |
Sigma-Aldrich |
D86256 |
|
| Chemical compound, drug |
All-trans retinoic acid(RA) |
Sigma-Aldrich |
R2625 |
|
| Chemical compound, drug |
Ketoconazole |
Tocris |
Tocris#1103 |
|
| Chemical compound, drug |
Cycloheximide |
Sigma-Aldrich |
C4859 |
|
| Chemical compound, drug |
Dexamethasone |
Sigma-Aldrich |
Sigma D4902 |
|
| Peptide, recombinant protein |
CAS9 |
PNA Bio |
CP01-20 |
|
| Sequence-based reagent |
X. tropicalis tbx5 exon5 sgRNA |
IDT DNA |
GGGGTTCTGATATGAAGTGA |
Steimle et al., 2018
|
| Sequence-based reagent |
X. laevis Tbx5 MO1 |
GeneTools |
5′-TTA GGA AAG TGT CTC TGG TGT TGC C -3′; |
Brown et al., 2005
|
| Sequence-based reagent |
X. laevis Tbx5 3 bp mismistach MO1 |
GeneTools |
5′-TCA GTA AAG TAT CTC TGG TGT TGC C-3′ |
This paper |
| Sequence-based reagent |
X. laevis Tbx5 MO2 |
GeneTools |
5′-CAT AAG CCT CCT CTG TGT CCG CCA T-3 |
Brown et al., 2005
|
| Sequence-based reagent |
X. laevis Tbx5 3 bp mismatch MO2 |
GeneTools |
5′-TAT CAG ACT CCT CTG TGT CCG CCA T-3′ |
This paper |
| Sequence-based reagent |
X. laevis Aldh1a2-MO |
GeneTools |
5′-GCA TCT CTA TTT TAC TGG AAG TCAT-3′ |
Strate et al., 2009
|
| Sequence-based reagent |
X. laevis Cyp26a1-MO |
GeneTools |
5′-TAG TGA GCA GAG TAT ACA GAT CCA T-3′ |
Janesick et al., 2013
|
| Sequence-based reagent |
X. laevis Cyp26c1-MO |
GeneTools |
5′-TAC AAG ATG TTC CTC CTT GAG ATC A-3′ |
Yu et al., 2016
|
| Commercial assay, kit |
Protein G-conjugated magnetic beads |
Life Technologies |
1,003D |
|
| Commercial assay, kit |
NEBNext Ultra DNA Library Prep Kit |
New England Biolabs |
E7370S |
|
| Commercial assay, kit |
Sera-Mag magnetic beads |
GE |
6515-2105-050-250 |
|
| Software, algorithm |
Morpheus |
Broad Institute |
https://software.broadinstitute.org/morpheusRRID:SCR_017386
|
|
| Commercial assay, kit |
SceI mega-nuclease enzyme |
New England Biolabs |
R0694S |
Use within 1 month of purchase, store at –80°C |
| Commercial assay, kit |
Dispase |
Corning Life Sciences |
354235 |
Use at 10 U/ml on Xenopus explants |
