Table 1. List of BACs and PCR primers for CHROMus mice.
| CHROMus ID | Transgenic line | Tissue/sensor type (effector [E] or sensor [S]) | Promoter or BAC | Genotyping primer 1 | Genotyping primer 2 |
|---|---|---|---|---|---|
| B3 | Acta2BAC-RCaMP1.07 | Smooth muscle – S | RP23-370F21 | GCTTGTCTGTAAGCGGATGCC | TGCTGCTGCCACTCTAGTGAGAAA |
| B4 | Myh6-GCaMP8 | Cardiac muscle – S | Addgene 55594 | AAGGGCGAGGAGCTGTTCA | CGATCTGCTCTTCAGTCAGTTGGT |
| B5 | Myh6-CatCh2_IRES_lacZ | Cardiac muscle – E | Addgene 55594 | GGAGATCTATGTGTGCGCTATC | CCAGCAGTTCTTCGACATCA |
| B6 | Acta2BAC-Optoα1AR_IRES_lacZ | Smooth muscle – E | RP23-370F21 | GGGTTGGTCCCGCTATATTC | GAAGGCAGGGATGGTCATAAA |
| B7 | Acta2BAC-Optoß2AR_IRES_lacZ | Smooth muscle – E | RP23-370F21 | ATAGCTCTCAGCAACCTGTTGGGT | TTCAACACGGTTTGGAGGCG |
| B8 | Acta2BAC-CatCh2_IRES_lacZ | Smooth muscle – E | RP23-370F21 | GGAGATCTATGTGTGCGCTATC | CCAGCAGTTCTTCGACATCA |
| B10 | Hcn4BAC-GCaMP8 | Cardiac conduction – S | RP23-281H22 | AAGGGCGAGGAGCTGTTCA | CGATCTGCTCTTCAGTCAGTTGGT |
| B14 | Acta2BAC-GCaMP_GR | Smooth muscle – S | RP23-370F21 | AAGGGCGAGGAGCTGTTCA | CGATCTGCTCTTCAGTCAGTTGGT |
| B15 | Gja5BAC-GCaMP_GR | Endothelium – S | RP24-255O4 | AAGGGCGAGGAGCTGTTCA | CGATCTGCTCTTCAGTCAGTTGGT |
| B16 | DbhBAC-CatCh2_IRES_lacZ | Sympathetic – E | RP23-354N13 | GGAGATCTATGTGTGCGCTATC | CCAGCAGTTCTTCGACATCA |
| B17 | Hcn4BAC-CatCh2_IRES_lacZ | Cardiac conduction – E | RP23-281H22 | GGAGATCTATGTGTGCGCTATC | CCAGCAGTTCTTCGACATCA |
| B19 | Acta2BAC-GCaMP2 | Smooth muscle – S | RP23-370F21 | AAGGGCGAGGAGCTGTTCA | CGATCTGCTCTTCAGTCAGTTGGT |
| B20 | Cdh5BAC-GCaMP8 | Endothelium – S | RP23-453P1 | AAGGGCGAGGAGCTGTTCA | CGATCTGCTCTTCAGTCAGTTGGT |
| B22 | LckBAC-Optoα1AR_IRES_lacZ | T cells – E | RP24-159E19 | GGGTTGGTCCCGCTATATTC | GAAGGCAGGGATGGTCATAAA |
| B23 | SftpcBAC-GCaMP8 | Alveolar – S | RP23-247J9 | AAGGGCGAGGAGCTGTTCA | CGATCTGCTCTTCAGTCAGTTGGT |
| B26 | Cdh5BAC-Optoα1AR_IRES_lacZ | Endothelium – E | RP23-453P1 | GGGTTGGTCCCGCTATATTC | GAAGGCAGGGATGGTCATAAA |
| B27 | Cdh5BAC-Optoß2AR_IRES_lacZ | Endothelium – E | RP23-453P1 | AACCTTGGAGGCTGGAAAGTAG | TTCAACACGGTTTGGAGGCG |
| B28 | Cdh5BAC-CatCh2_IRES_lacZ | Endothelium – E | RP23-453P1 | GGAGATCTATGTGTGCGCTATC | CCAGCAGTTCTTCGACATCA |
| B34 | Acta2BAC-GCaMP8.1_mVermilion | Smooth muscle – S | RP23-370F21 | AAGGGAGAGGAGCTGTTCA | CGATCTGCTCCTCTGTCAGCTGGT |
| B35 | Foxj1BAC-GCaMP8.1 | Ciliated epithelia – S | RP23-294J17 | AAGGGAGAGGAGCTGTTCA | CGATCTGCTCCTCTGTCAGCTGGT |
| B36 | Aldh1l1BAC-Optoα1AR_IRES_lacZ | Glia – E | RP23-7M9 | GGGTTGGTCCCGCTATATTC | GAAGGCAGGGATGGTCATAAA |