TABLE 1.
Yeast strain genotypes and construction
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| 479-2A | MATα acc1cs leu2-3,112 trp1-1 ura3-1 ade2-1 his3-11,15 can1-100 | H. Klein |
| 485-13A | MATa acc1cs leu2-3,112 ura3-1 ade2-1 his3-11,15 can1-100 | H. Klein |
| LWY3682 | MATα leu2-3,112 lys2-801 ura3-52 his3-Δ200 suc2-Δ9 trp1-Δ901 vac8::HIS3 pYW40 (URA3) | L. Weisman (52) |
| YB336 | MATa nmt1-181 leu2-3,112 ura3-52 ade2-101 his3Δ200 lys2-801 | Duronio et al. (9) |
| W303d | MATa/MATα leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 ade2-1/ade2-1 his3-11,15/his3-11,15 can1-100/can1-100 | R. Rothstein |
| YRXS12 | MATα acc1ts leu2-Δ1 ura3-52 ade2-101 lys2-801 his3-Δ200 | Schneiter et al. (40) |
| 001 | MATa/MATα ACC1/acc1cs leu2-3,112/leu2-3,112 TRP1/trp1-1 ura3-1/ura3-1 ade2-1/ade2-1 his3-11,15/his3-11,15 can1-100/can1-100 | This work |
| 003 | MATa/MATα ACC1/acc1::TRP1 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 ade2-1/ade2-1 his3-11,15/his3-11,15 can1-100/can1-100 | This work |
| 009 | MATa/MATα acc1cs/acc1cs leu2-3,112/leu2-3,112 TRP1/trp1-1 ura3-1/ura3-1 ade2-1/ade2-1 his3-11,15/his3-11,15 can1-100/can1-100 | This work |
| 011-2 | MATa/MATα acc1::TRP1/acc1cs leu2-3,112/leu2-3,112 TRP1/trp1-1 ura3-1/ura3-1 ade2-1/ade2-1 his3-11,15/his3-11,15 can1-100/can1-100 | This work |
| YRXS301 | MATa/MATα ACC1/acc1::TRP1 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 ade2-1/ade2-1 his3-11,15/his3-11,15 can1-100/can1-100 pRXS53 (URA) pRXS89 (HIS) pCG002::Tn10-LUK#21 (LEU) | This work, strain 003 transformed with pRXS53, pRXS89, and pCG002::Tn10-LUK#21 |
| YRXS301 | MATa/MATα acc1ts/acc1cs leu2-Δ1/leu2-3,112 ura3-52/ura3-1 ade2-101/ade2-1 LYS2/lys2-801 TRP1/trp1-1 his3-Δ200/his3-11,15 CAN1/can1-100 | This work, diploid from cross between YRXS12 and 485-13A |
| YRXS303 | MATa VAC8-GFP-KanMX6 ura3-52 his3Δ200 | This work |
| YRXS304 | MATα acc1cs VAC8-GFP-KanMX6 leu2-3,112 ura3-1 ade2-1 trp1-1 his3-11,15 can1-100 | This work |
| YRXS305 | MAT? acc1cs vac8::HIS3 leu2-3,112 ura3 ade2-1 trp1 his3 pYW40 (URA3) | This work |
| YRXS306 | MAT? acc1cs nmt1-181 leu2-3,112 ura3 ade2 lys2-801 his3 | This work |