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. 2021 Oct 29;12:6242. doi: 10.1038/s41467-021-26495-2

Fig. 9. In vivo application of BC-11 or Sitagliptin improves collagen alignment and fiber orientation in mouse scars.

Fig. 9

A Picrosirius red staining and immunofluorescent staining of B Col3a1 and C fibronectin in mouse skin and scars are shown. Four mice per group were analyzed. Arrows indicate areas of increased matrix density. D H&E images of mouse skin and scars. Squares indicate areas analyzed for collagen alignment. Histograms illustrate measurement of fiber orientation. E Calculation of alignment coefficient by CurveAlign in mouse skin and scar. N = 4 mice were analyzed per group, and three to four Regions of interest were calculated per image. Whiskers represent range maximum and minimum values with <1.5 interquartile range, boxes represent 25th–75th quartiles, line represents mean. Statistical significance was tested using one-way ANOVA with Tukey post-test. Skin vs Scar untreated p < 0.0001; Scar untreated vs Scar+BC-11 p = 0.0018; Scar untreated vs Scar+Sitagliptin p = 0.551. This mouse experiment was performed once, repetition of the experiment was not permitted by the ethics committee. NS p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source data file.