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. 2021 Oct 29;4:1240. doi: 10.1038/s42003-021-02766-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Repeats from tcTRP9 and tcTRP24 are shown with darker shades and lighter shades, respectively. a Alignment of the sequences of an individual repeats from each of the two designs. Both designs are based on a 57-residue sequence that forms an anti-parallel two-helix bundle. The overall sequence identity (noted by asterisks) is 37%; sequence similarity (including D/E, N/Q, K/R, and I/V pairs) is 53%. Many of the identities between designs are observed at less-constrained surface-exposed positions, but also are found at interfacial positions that contribute to packing between helices and repeats. b Superposition of individual repeats from tcTRP9 and tcTRP24 gives an overall rmsd across all alpha carbons of approximately 1.5 Å, indicating that the backbone architecture of individual repeats from the two designs is similar. c, d Superposition of a single repeat from tcTRP9 and tcTRP24 (dark and light blue, respectively) and extension to the neighboring immediate downstream repeat (dark and light red, respectively) illustrates that the curvature and diameters between the two constructs are facilitated by altered packing angles between sequential repeats (resulting in an alpha-carbon rmsd value between the downstream repeats of over 4 Å).