Table 2.
Cryo-EM data collection, refinement, and validation statistics.
| tcTRP248SS (EMD-24425) (PDB 7RDR) | |
|---|---|
| Data collection and processing | |
| Magnification | 38 Kx |
| Voltage (kV) | 200 |
| Electron exposure (e–/Å2) | 40 |
| Defocus range (μm) | −0.8 to −2.3 |
| Pixel size (Å) | 1.16 |
| Symmetry imposed | C3 |
| Initial particle images (no.) | 627763 |
| Final particle images (no.) | 121426 |
| Map resolution (Å) | 6.9 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 30–6.9 |
| Refinement | |
| Initial model used (PDB code) | tcTRP248SS |
| Model resolution (Å) | 6.9 |
| FSC threshold | 0.143 |
| Model resolution range (Å) | 30–6.9 |
| Map sharpening B factor (Å2) | −418.6 |
| Model composition | |
| Non-hydrogen atoms | None |
| Protein residues | 1368 |
| Ligands | None |
| B factors (Å2) | |
| Protein | 20.0 |
| Ligand | N/A |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.01 |
| Bond angles (°) | 1.2 |
| Validation | |
| MolProbity score | 1.6 |
| Clashscore | 1.05 |
| Poor rotamers (%) | 0 |
| Ramachandran plot | |
| Favored (%) | 98.32 |
| Allowed (%) | 1.68 |
| Disallowed (%) | 0 |