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. 2021 Oct 29;12(11):1023. doi: 10.1038/s41419-021-04308-0

Fig. 5. The expression of Il6 after knockdown or overexpression of Etv5 in macrophages.

Fig. 5

A BMDMs were transfected with siRNA negative control (siRNA NC) or siRNA specific to Etv5 (siRNA Etv5) 48 h and Il6 expression was assessed by RT-qPCR 48 h later. Il6 expression levels of Raw264.7 cells after knockdown of Etv5 using shRNA B or overexpression of Etv5 C. Il6 expression levels of Raw264.7 cells after knockdown of Etv5 using shRNA D or overexpression of Etv5 E under MMe induction condition. F Il6 expression levels of BMDMs after treatment with palmitate, glucose, and insulin alone or with a combination of these compounds for 24 h. G–I Measuring IL-6 protein levels in the supernatant of Raw264.7 cell culture by ELISA. Cells were treated with palmitate (P) or PGI G, or underwent Etv5 knockdown using siRNA H, or shRNA I. Data in this result were all representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test.