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. 2021 Oct 29;11:21304. doi: 10.1038/s41598-021-00706-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic for EAC PDO generation. Diagnostic biopsies are enzymatically and mechanically dissociated into a single cell suspension, which is embedded in a basement membrane extract drop and grown in specialized culture medium. The PDOs are characterized by a pathologist to confirm EAC histology. Once sufficiently expanded and cryopreserved, the lines can be used for drug screening assays. Created with BioRender.com.