| Antibodies |
| Guinea pig anti-Vglut1 (1:1000) |
Sigma |
AB5905 |
| Rabbit anti-Homer1 (1:500) |
CedarLane |
160003SY |
| Goat anti-mCherry (1:500) |
CedarLane |
AB0040-200 |
| Chicken anti-GFP (1:500) |
Aves |
GFP-1020 |
| Rabbit anti-tRFP (1:500) |
Evrogen |
AB233 |
| Rabbit anti-S100 (1:300) |
VWR/ProteinTech |
15146-1-AP |
| Chick anti-Neurofilament 200 kDa (1:500) |
Aves |
NFH |
| Donkey anti-Guinea pig Alexa Fluor 647 (1:500) |
Jackson Immunoresearch |
706-605-148 |
| Donkey anti-Rabbit Alexa Fluor 488 (1:1000) |
ThermoFisher |
A-21206 |
| Donkey anti-Goat IgG Alexa Fluor 546 (1:1000) |
LifeTech |
A11056 |
| Donkey anti-Chicken IgG Alexa Fluor 488 (1:1000) |
Jackson Immunoresearch |
703-545-155 |
| Donkey anti-Rabbit IgG DyLight 405 (1:1000) |
Jackson Immunoresearch |
711-475-152 |
| Bacterial and Virus Strains |
| AAV2-retro-hSyn-Cre-WPRE |
Addgene |
105553-AAVrg |
| AAV2-retro-synaptophysin-mScarlet |
Penn Viral Core |
n/a |
| AAV2-retro-synaptophysin-tdTomato |
Penn Viral Core |
n/a |
| rAAV2-retro-hSyn-H2B-GFP-bGH |
Boston Children’s Hospital Viral Core |
n/a |
| AAV2-retro-hSyn-H2B-mTagBFP2-bGH |
Boston Children’s Hospital Viral Core |
n/a |
| AAV2/1-hSyn-FlpO |
Boston Children’s Hospital Viral Core |
n/a |
| Chemicals, Peptides, and Recombinant Proteins |
| urethane |
Sigma |
U2500 |
| Experimental Models: Organisms/Strains |
|
TauFSFiAP
|
This study |
n/a |
|
R26-PhiC31o
|
JAX |
#007670 |
|
R26FSF-LSL-ReaChR
|
JAX |
024846 |
|
R26FSF-ReaChR
|
derived from JAX#024846 |
n/a |
|
Brn3acKOAP
|
Badea et al., 2009a
|
n/a |
|
R26IAP
|
Badea et al., 2009b
|
n/a |
|
Gad2T2A-NLS-mCherry
|
JAX |
023140 |
|
Cdx2-Cre
|
Coutaud and Pilon, 2013
|
n/a |
|
AdvillinFlpO
|
Choi et al., 2020
|
n/a |
|
Rosa26FSF-LSL-tdTomato(Ai65) |
JAX |
021875 |
|
Rosa26LSL-synaptophysin-tdTomato (Ai34) |
JAX |
012570 |
|
RetCreER
|
Luo et al., 2009
|
n/a |
|
TrkCCreER
|
Bai et al., 2015
|
n/a |
|
TrkBCreER
|
Rutlin et al., 2014
|
n/a |
|
PtgfrCreER
|
This study; to be described elsewhere |
n/a |
|
PvalbT2A-FlpO
|
JAX |
022730 |
|
Piezo2−
|
Nonomura et al., 2017
|
n/a |
|
Piezo2fl
|
JAX |
027720 |
| Oligonucleotides |
| GGATGGGAAACTGAGGCTCT |
Integrated DNA Technologies |
fwd - TauFSFiAP
|
| ATGGTGGCGAATTCCAAATCA |
Integrated DNA Technologies |
rev - TauFSFiAP
|
| Software and Algorithms |
| Imaris |
Bitplane |
RRID:SCR_007370 |
| Matlab |
Mathworks |
https://www.mathworks.com/products/matlab.html; RRID: SCR_001622 |
| ImageJ |
NIH |
https://imagej.nih.gov/ij/; RRID: SCR_003070 |
| Python |
Van Rossum, G., & Drake Jr, F. L. (1995)
|
https://www.python.org/
|
| Other |
| NAIR hair removal cream |
Church and Dwight Co.; Princeton, NJ |
n/a |
| Ti:Sapphire laser |
Coherent |
Vision S |
| CCD Camera |
Rolera |
Bolt |
| Stereotaxic apparatus |
KOPF |
Model 920 |
| Cannulae |
Microgroup |
n/a |
| UV-cured adhesive |
KOA |
n/a |
| Vetbond |
3M |
1469SB |
| C&B Metabond |
Parkell |
S380 |
| Galvo mirrors |
Cambridge Technologies |
6210H |
| F-theta lens |
Thorlabs |
FTH100-1064 |
| Multielectrode arrays |
Cambridge Neurotech |
Edge32, Edge64, and H6b |
| RHD USB interface board |
Intan Technologies |
C3100 |
| Multiphoton microscope |
Scientifica |
n/a |
| 16X water immersion objective |
Nikon |
CFI75 |
| Patch-clamp amplifier |
Molecular Devices |
Multiclamp 700A |
