Fig. 6. MYH9_761-768 immunization exacerbates EAE disease course.

C57BL/6 mice were immunized with 100 µg MOG_35-55, admixed with OVA_257-264 (n = 10) or with MYH9_761-768 (n = 9), and received 200 ng B. pertussis Toxin (PTX) i.p. at days 0 and 2. A EAE score and changes in body weight were monitored. *P < 0.05, **P < 0.01, by Mann–Whitney test. B, C Mice were sacrificed at day 29 and flow cytometry analysis was performed in spleens (SPL) and central nervous system (CNS). B Percentages of total CD4⁺ and CD8⁺ T cells (upper panels), and of CD44⁺ CD4⁺ or CD8⁺ T cells (lower panels), in the CNS of mice immunized with MOG_35-55 plus OVA_257-264 (blue) or plus MYH9_761-768 (red). *P < 0.05, by Mann–Whitney test. C Percentages of T naïve (T_N), T virtual memory (T_VM), and T memory (T_M) in gated CD8⁺ T cells from SPL or CNS of mice immunized with MOG_35-55 plus OVA_257-264 or plus MYH9_761-768. The data are from a single experiment representative of two independent experiments. Bars represent means ± SEM.