Skip to main content
. 2021 Oct 29;12(11):1016. doi: 10.1038/s41419-021-04274-7

Fig. 1. TM induces ER stress, autophagy, fibrosis and apoptosis in mouse kidneys.

Fig. 1

C57BL/6 mice (male, 8–10 weeks) were subjected to two weekly injections of 0.25 mg/kg TM (or DMSO for control) to collect kidney tissues 7 days or 14 days later. A, B Immunoblot analysis of proteins in kidney cortical tissues to indicate ER stress and autophagy. For quantification, the proteins bands were analyzed by densitometry and their signals were normalized with that of GAPDH and values expressed relative to mean of control counterparts. C Representative images and quantification of the staining of LC3. Bar = 20 μm. D, E Immunoblot analysis of fibrosis marker proteins and cleaved caspase-3 in kidney cortical tissues. For quantification, the proteins bands were analyzed by densitometry and their signals were normalized with that of GAPDH and values expressed relative to mean of control counterparts. F Representative images and quantification of the staining of fibronectin (FN), Masson trichrome, and TUNEL assay. Bar = 100 μm. Quantitative data are expressed as mean ± SD. n = 6. **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. control.