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. 2021 Oct 29;12(11):1016. doi: 10.1038/s41419-021-04274-7

Fig. 3. Inhibition of autophagy attenuates TM -induced fibrosis and apoptosis in HK-2 cells.

Fig. 3

HK-2 cells were treated with or without TM (100 nM) in the absence or presence of chloroquine (CQ, 20 μM) for 24 h. A, B Cell lysates were analyzed for LC3 and p62 by immunoblot analysis and quantified by densitometry. C Representative phase-contrast images and TUNEL staining of HK-2 cells. Scale bar = 100 μm. D Counting of TUNEL positive cells. E–F Cell lysates were analyzed for levels of FN, Collagen I, and Cleaved Caspase-3 by immunoblot analysis and quantified by densitometry. G Representative images of Collagen I immunofluorescence staining. Scale bar = 100 μm. H Quantitative analysis of Collagen I immunofluorescence. Quantitative data are expressed as mean ± SD. n = 4. *p < 0.05, **p < 0.01.