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. 2021 Oct 29;12(11):1016. doi: 10.1038/s41419-021-04274-7

Fig. 5. Inhibition of ER stress attenuates TGF-β1-induced autophagy, fibrosis and apoptosis in HK-2 cells.

Fig. 5

HK-2 cells were treated with or without TGF-β1 (5 ng/ml) for 24 h in the absence or presence of 4-phenyl butyric acid (4-PBA,1 mM). A Representative immunoblot analysis of p-PERK, PERK, and β-actin. B Densitometric analysis of p-PERK band intensity. C Representative phase-contrast and TUNEL staining images. Scale bar = 100 μm. D Counting of TUNEL staining positive cells. E Cell lysates were analyzed for LC3, FN, Collagen I, and cleaved Caspase-3 by immunoblot. F Densitometric analysis of LC3-II and cleaved caspase-3. G Representative images of Collagen I immunofluorescence staining. Scale bar = 100 μm. H Quantitative analysis of Collagen I immunofluorescence intensity. Data are expressed as mean ± SD. n = 4. *p < 0.05, **p < 0.01.