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. 2021 Oct 29;12(11):1016. doi: 10.1038/s41419-021-04274-7

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Fig. 7 — HK-2 cells were treated with or without TGF-β1 (5 ng/ml) for 24 h in the absence or presence of 1 μM GSK2656157. A Cell lysates were analyzed for levels of p-PERK, PERK, p-eIF2α, eIF2α, and β-actin by immunoblot analysis. B Densitometric analysis of p-PERK and p-eIF2α immunoblots relative to β-actin. C Representative phase-contrast and TUNEL staining images. Scale bar = 100 μm. D Counting of TUNEL-staining positive cells. E Cell lysates were analyzed for LC3, FN, Collagen I, cleaved Caspase-3, and β-actin by Immunoblot analysis. F Densitometric analysis of LC3-II and cleaved Caspase-3 blots relative to β-actin. G Representative images of Collagen I immunofluorescence staining. Scale bar = 100 μm. H Quantitative analysis of Collagen I immunofluorescence intensity. Data are expressed as mean ± SD. n = 4. *p < 0.05; **p < 0.01.