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. 2021 Oct 29;12(11):1016. doi: 10.1038/s41419-021-04274-7

Fig. 8. Inhibition of autophagy exaggerates ER stress during TM or TGF-β1 treatment of HK-2 cells.

Fig. 8

HK-2 cells were treated with or without 100 nM TM A, B or 5 ng/ml TGF-β1 CF for 24 h in the absence or presence of 20 μM CQ AD or 10 mM 3-methyladenine EF. Cell lysates were analyzed for BiP, p-eIF2α, eIF2α and GAPDH/β-actin A, C, E. B, D, F Quantitative analysis of the intensity of BiP and p-eIF2α by densitometry. Data are expressed as mean ± SD. n = 4. *p < 0.05; **p < 0.01.