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. 2021 Oct 29;12(11):1027. doi: 10.1038/s41419-021-04307-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Fe L3-edge NEXAFS (near edge X-ray absorption fine structure) spectrum was acquired from two absorption cellular images scanned at two energies, E1 (709 eV) and E2 (705 eV) above and below the absorption edge of Fe, respectively. B Evaluation of Fe distribution and content using scanning transmission X-ray microscopy. C Endogenous Fe levels within the pulmonary epithelial cells of the OGD/R model, as detected by Fe assay. D Evaluation of the levels of ferroptosis-related proteins under different conditions. E Quantification of the examined proteins. F Evaluation of ferritin and ferroportin levels (red) in lung tissues, using immunohistochemistry. Nuclear staining done with DAPI (blue) after OGD/R. Scale bar: 100 μm. G Cell survival after OGD/R induction and the opposite effect with Fer-1 (0.1 μM). H GSH levels under different conditions. I MDA levels under different conditions. Error bars denote standard error from three individual experiments. Data are expressed as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; *relative to control; ^#relative to OGD/R.