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. 2021 Oct 29;7:324. doi: 10.1038/s41420-021-00719-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Representative western blot analysis of NLRP3 (23 kDa) protein in injured kidneys at indicated time points; GAPDH was used as a loading control. c, d Relative expression of NLRP3 mRNA in kidneys in injured kidneys at indicated time points. e Serum levels of NLRP3 from mild and severe group mice. ***P < 0.001 means significant versus baseline. f Urine levels of NLRP3 from mild and severe group mice. ***P < 0.001 means significant versus baseline. n = 3 mice for the sham group, n = 5 for mild and severe AKI per time-point group, respectively. Data are mean ± SD. **P < 0.01, ***P < 0.001, ****P < 0.0001. ns not significant.