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TNBS-induced CGRP release to the spinal cord was characterized by immunostaining (A-D) showing that β2AR inhibition but not activation blocked CGRP release (E). CGRP levels in the spinal cord were also characterized by slot blot (F) showing that TNBS evoked CGRP increase which was blocked by β2AR inhibition but not activation (G). n=3. p<0.01.
