Table 2.

Effects of the nano-chitosan on the proliferation of Acanthamoeba trophozoites and cysts

Time	Concentrations	Trophozoite Mean ± SD	Cyst Mean ± SD	P-value
24 h	12.5 µL/mL	57.07 ± 2.186	80.55 ± 4.811	0.002
	25 µL/mL	48.48 ± 4.656	68.37 ± 1.480	0.002
	50 µL/mL	38.88 ± 4.811	57.50 ± 3.859	0.006
	100 µL/mL	27.68 ± 2.906	55.57 ± 2.414	0.000
	200 µL/mL	19.96 ± 4.050	39.92 ± 2.537	0.002
	Control −	99.00 ± 1.732	99.33 ± .667	0.795
	Control +	10.47 ± 4.268	40.99 ± 2.273	0.000
48 h	12.5 µL/mL	41.16 ± 4.566	61.16 ± 2.670	0.003
	25 µL/mL	32.47 ± 1.480	52.56 ± 2.220	0.000
	50 µL/mL	26.19 ± 4.123	40.99 ± 2.273	0.006
	100 µL/mL	17.94 ± 4.441	32.32 ± 4.628	0.018
	200 µL/mL	7.72 ± 0.595	19.30 ± 3.350	0.004
	Control −	97.33 ± 2.516	99.00 ± 1.00	0.398
	Control +	0.000 ± 0.000	15.44 ± 1.191	0.000
72 h	12.5 µL/mL	23.71 ± 1.110	38.38 ± 6.308	0.017
	25 µL/mL	15.44 ± 1.191	31.36 ± 1.745	0.000
	50 µL/mL	5.34 ± 4.637	25.75 ± 1.312	0.002
	100 µL/mL	00.00 ± 0.000	18.80 ± 3.700	0.013
	200 µL/mL	00.00 ± 0.000	2.56 ± 4.441	0.423
	Control −	97.00 ± 2.645	97.67 ± 1.202	0.749
	Control +	0.000 ± 0.000	0.000 ± 0.000	0.000