Table 6.
Summary of the metabolites that changed significantly in the livers of growing heifers fed a Lys-adequate diet through RPLC-MS analysis.
| Metabolites1 | ID2 | Formula | Mode3 | VIP | P-value | FC4 | Direction5 |
|---|---|---|---|---|---|---|---|
| Arachidic acid | HMDB02212 | C20H40O2 | – | 1.47 | 0.027 | 0.63 | ↓ |
| 11Z-Eicosenoic acid | HMDB02231 | C20H38O2 | – | 1.44 | 0.043 | 0.74 | ↓ |
| PC (16:0/20:4(8Z, 11Z, 14Z, 17Z)) | HMDB08462 | C44H80NO8P | + | 1.39 | 0.048 | 1.54 | ↑ |
| PC (16:0/18:2(9Z, 12Z)) | HMDB08133 | C42H80NO8P | + | 1.17 | 0.049 | 1.28 | ↑ |
| PC (16:0/20:3(8Z, 11Z, 14Z)) | HMDB07981 | C44H82NO8P | + | 1.16 | 0.015 | 1.41 | ↑ |
| PC (18:0/20:4(8Z, 11Z, 14Z, 17Z)) | HMDB08049 | C46H84NO8P | + | 1.23 | 0.049 | 1.47 | ↑ |
| PC (18:0/20:3(5Z, 8Z, 11Z)) | HMDB08046 | C46H86NO8P | + | 1.39 | 0.012 | 1.59 | ↑ |
| Cer (d18:1/24:1(15Z)) | HMDB04953 | C42H81NO3 | + | 1.11 | 0.025 | 1.61 | ↑ |
| Cer (d18:1/22:0) | HMDB04952 | C40H79NO3 | + | 1.10 | 0.021 | 1.59 | ↑ |
| Cer (d18:1/23:0) | HMDB00950 | C41H81NO3 | + | 1.02 | 0.047 | 1.47 | ↑ |
RPLC-MS = reversed-phase liquid chromatography-mass spectrometry; VIP = variable important in projection; FC = fold change; PC = phosphatidylcholine; Cer = ceramide.
The different metabolites were filtered using significance estimate of P < 0.05 and VIP > 1.0 (n = 5). LD group: growing heifers fed a Lys-deficient diet; LA group: growing heifers fed a Lys-adequate diet.
Compound ID of metabolites in human metabolome database.
‘-’ indicates negative mode and ‘+’ indicates the positive mode.
Fold change indicates the relative amounts of LA group compared with LD group.
‘↑’ indicates the metabolite in LA group was increased when compared with LD group; ‘↓’ indicates the metabolite in LA group was decreased when compared with LD group.