Table 8.
Enrichment of the KEGG pathway using significantly changed metabolites from the combined HILIC-MS and RPLC-MS analyses.
| KEGG Pathway name | -log (P-value)1 | Impact value2 |
|---|---|---|
| Sphingolipid metabolism | 2.410 | 0.294 |
| Alanine, aspartate and glutamate metabolism | 2.449 | 0.265 |
| Histidine metabolism | 4.525 | 0.140 |
| Glycerophospholipid metabolism | 1.988 | 0.101 |
| Aminoacyl-tRNA biosynthesis | 6.150 | 0.056 |
| Valine, leucine and isoleucine degradation | 1.965 | 0.022 |
| Valine, leucine and isoleucine biosynthesis | 2.336 | 0.013 |
| Nitrogen metabolism | 4.759 | <0.001 |
KEGG = Kyoto Encyclopedia of Genes and Genomes; HILIC-MS = hydrophilic interaction liquid chromatography-mass spectrometry; RPLC-MS = reversed-phase liquid chromatography-mass spectrometry.
1
The KEGG pathway P-values were calculated by comparing the proportion of metabolites in this pathway.
2
The impact value is calculated by adding up the importance measures of each of the matched metabolites and then dividing by the sum of the important measures of all metabolites in each pathway.