Table 2.
Information of primers.
| Gene | Accession number | Primer sequence (5′-3′) | Product length, bp |
|---|---|---|---|
| Inppl1 | NM_017349797 | F: CAGAGCACGAGAACCGCATCAG | 199 |
| R: AGCCGCAGGATGTCCAGGTAG | |||
| Inpp4b | NM_008267416 | F: TCCTAAGAGCACAGCGGAGAGC | 195 |
| R: GCTGCCTTCACTGCCACCATC | |||
| Frk | XM_008263392 | F: CAAGCGATGGCCTCTGTGTCAG | 195 |
| R: CTGCTACTGGAGTGGTGTTGTTCC | |||
| Phlda3 | XM_002717603 | F: GCGGCGGCGAGATTGACTTC | 101 |
| R: CTGGATGGCCTGCTGGTTCTTG | |||
| Wnt10b | NM_002711076 | F: TGTGCCATCCCTCTTCCTTA | 150 |
| R: GGCTCCACCTCTAACTTCTGC | |||
| CTNNB1 | DQ786777 | F: TTCTTGGGACTCTTGTTCAGC | 122 |
| R: CACTTGGCACACCATCATCT | |||
| GSK-3β | NM_001146156 | F: ATCCATGTCTCCCTGTCCAC | 119 |
| R: TTTCCTCTTCCCACTCCTGA | |||
| DKK1 | NM_001082737 | F: ATTCCAACGCCATCAAGAAC | 163 |
| R: CCACACTCCTCGTCCTCTGT | |||
| Notch1 | XM_011518717 | F: TGCGAGACCAACATCAACGAGTG | 94 |
| R: TCAGGCAGAAGCAGAGGTAGGC | |||
| JAG1 | XM_018261778 | F: TGGAGGAGGACGACATGGACAAG | 176 |
| R: CATCCGATTGAGGCTCTGTGCTG | |||
| Hes1 | XM_002716517 | F: CCAGATCAACGCCATGACCTATCC | 200 |
| R: ACACCTTAGCCGCCTCTCCAG | |||
| Hes5 | NM_008253710 | F: AGACCGCATCAACAGCAGCATC | 105 |
| R: ATCTCCAGGATGTCCGCCTTCTC | |||
| BMP2 | XM_001082650 | F: GACATCCTGAGCGAGTTCGAGTTG | 113 |
| R: CGGCGGTACAAGTCCAGCATG | |||
| BMP4 | NM_001195723 | F: CTAAGCATCACCCACAGCGG | 163 |
| R: CAGTCATTCCAGCCCACGTC | |||
| TGF-β1 | NM_008249704 | F: 5′- CCGTTTCTTTCGTGGGATAC | 108 |
| R: GGTAAGGGAGGAGGGTCTCA | |||
| GAPDH | NM_001082253 | F: TGCCACCCACTCCTCTACCTTCG | 118 |
| R: CGAAGGTAGGGATGGGTGGCA |
Inppl1 = inositol polyphosphate phosphatase like 1; F = forward primer; R = reverse primer; Frk = fyn-related kinase; Phlda3 = pleckstrin homology like domain family A member 3; CTNNB1 = catenin beta 1; GSK-3β = glycogen synthase kinase 3 beta; DKK1 = dickkopf-1; JAG1 = Jagged1; BMP = bone morphogenetic protein; TGF-β1 = transforming growth factor-β; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.