| Step | Solution | Time of immersion |
|---|---|---|
| 1 | 80% ethanol containing 1% NaOH | 5 min |
| 2 | 70% ethanol | 2 min |
| 3 | 50% ethanol | 2 min |
| 4 | 30% ethanol | 2 min |
| 5 | 10% ethanol | 2 min |
| 6 | ddH2O | 2 min, twice |
| 7 | 0.06% potassium permanganate | 10 min |
| 8 | ddH2O | 2 min, twice |
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