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. Author manuscript; available in PMC: 2021 Oct 30.
Published in final edited form as: Mol Cell. 2020 Dec 9;81(2):239–254.e8. doi: 10.1016/j.molcel.2020.11.006

Figure 4. ZNF410 Binding to the CHD4 Locus Occurs at Highly Conserved Motif Clusters.

Figure 4.

(A) ChIP-seq profiles of endogenous ZNF410, HA-ZNF410, and H3K27ac. The CHD4 promoter and enhancer are highlighted in orange. ZNF410 binding motifs are denoted by vertical black lines at the bottom. The 8 peak-associated genes are shown below the tracks. ZNF410 knockout (KO) cells and cells transduced with empty vector serve as negative controls. Pro, proximal; dis, distal; HA-ZNF410, N-terminal HA-tagged ZNF410; HA, hemagglutinin.

(B) Browser tracks of endogenous ZNF410 ChIP-seq occupancy at the 7 murine counterparts in differentiated mouse erythroid cells. ZNF410 binding motifs are shown at the bottom. The immunoglobulin G (IgG) track served as a negative control.

(C) Summary of ZNF410 binding motif counts at the 8 peaks and derived de novo motif logo in the human genome. Red font indicates the variants.

(D and E) mRNA levels of the 7 ZNF410-bound genes in HUDEP-2 cells transduced with the indicated sgRNAs (D) and primary erythroblasts electroporated with the indicated sgRNAs (E) by RT-qPCR (n = 2). Results are shown as mean ± SD.