TABLE 3.
Differential activation of PKA by cAMP-elevating agents in neural crest cultures
| Agent | % of total endogenous PKA activateda
|
|||
|---|---|---|---|---|
| Expt 1 | Expt 2 | Expt 3 | Avg ± SEM | |
| Control | 2.0 | 2.4 | 0.2 | 1.5 ± 0.7 |
| IBMX (100 μM) | 4.4 | 0.0 | 1.8 | 2.0 ± 1.3 |
| Ro 20-1724 (20 μM) | 0.4 | 6.5 | 4.7 | 3.9 ± 1.8 |
| Forskolin (10 μM) | 8.5 | 6.0 | 13.4 | 9.3 ± 2.2 |
| 8-Br-cAMP (100 μM) | 56.1 | 58.3 | 30.0 | 48.1 ± 9.1 |
a
Cultures for PKA assays were prepared as described in Materials and Methods. cAMP-elevating agents were applied as indicated to crest cells for 2 h and processed to measure the percentage of total endogenous PKA activated. Data represent the average of duplicate radiometric values and three independent experiments.