. 2021 Oct 27;9:e12399. doi: 10.7717/peerj.12399

Table 2. In vitro shoot regeneration, growth, rooting and acclimatization of tetraploids, mixoploids and octoploids of N. cadamba.

Morphological characteristics	Tetraploid	Mixoploid	Octoploid
Regeneration
Number of shoots ± SE	5.4 ± 0.1^b	4.3 ± 0.2^a	4.2 ± 0.3^a
Regeneration (%) ± SE	94.4 ± 5.6^b	100.0 ± 0.0^b	83.3 ± 0.0^a
Growth
Stem height (cm) ± SE	7.2 ± 0.0^c	6.8 ± 0.0^b	4.4 ± 0.4^a
Number of leaves ± SE	12.8 ± 0.2^a	12.9 ± 0.2^a	13.0 ± 0.3^a
Number of nodes ± SE	6.4 ± 0.1^a	6.4 ± 0.1^a	6.5 ± 0.0^a
Stem diameter (mm) ± SE	2.3 ± 0.1^a	2.7 ± 0.1^b	2.9 ± 0.1^b
Rooting
Root length (cm) ± SE	1.8 ± 0.1^c	1.6 ± 0.0^b	1.4 ± 0.0^a
Number of roots ± SE	13.8 ± 0.9^b	16.1 ± 0.5^b	11.1 ± 0.7^a
Rooting (%) ± SE	100.0 ± 0.0^a	100.0 ± 0.0^a	100.0 ± 0.0^a
Acclimatization percentage
Jiffy-7 ± SE	94.4 ± 5.6^b	94.4 ± 5.6^b	94.4 ± 5.6^b
Loamy soil ± SE	88.9 ± 5.6^b	94.4 ± 5.6^b	88.9 ± 5.6^b
Loamy soil: compost 1:3 ± SE	50.0 ± 9.6^a	61.1 ± 5.6^a	66.7 ± 0.0^a

Notes.

For each parameter, means with different letters indicate significantly different at 5% level of probability using Duncan’s test (p < 0.05).