Table 3.

Effect of diet, time collection and their interaction on ruminal microbiota (copies log₁₀/g) in ruminal fluid collected from Holstein calves fed a control diet (CTR) and a nitrate-containing diet (NIT).

Item	Diet		SEM	P-value
	CTR	NIT		Diet	Time	D × T
Total bacteria	11.8	11.4	0.03	0.001	0.002	0.353
Methanogens	8.4	8.2	0.03	0.048	0.001	0.013
Fungi	4.8	6.5	0.12	0.001	0.122	0.003
Protozoa, log10/mL	4.8	6.0	0.04	0.001	0.057	0.140
Fibrobacter succinogenes	6.4	7.2	0.07	0.001	0.460	0.747
Ruminococcus albus	7.4	6.5	0.06	0.001	0.003	0.182
Ruminococcus flavefaciens	9.1	7.4	0.05	0.001	0.051	0.061
Selenomonasruminatium	8.5	7.7	0.06	0.001	0.160	0.046
Veillonellaparvula	5.3	5.2	0.08	0.349	0.110	0.800

D × T = Diet × Time interaction.