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. 2021 Oct 30;21:301. doi: 10.1186/s12866-021-02352-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Enrichment of Fusobacterium nucleatum in ESCC detected by FISH. Graph A: ESCC specimens stained with DAPI. Cell nuclei were stained in blue; B: ESCC specimens stained with both DAPI and a universal bacterial probe (EUB338). Bacterial conserved regions were stained in green; C: ESCC specimen stained with both DAPI and a Fusobacterium-specific probe (FUSO). The F. nucleatum-specific regions were stained in red; D: ESCC specimens triply stained with DAPI, FUSO and EUB338. Cell nuclei (in blue), bacterial conserved regions (in green) and F. nucleatum-specific regions (in red) were clearly visible (all × 400)