Table 6.

Sequences of oligonucleotide primers and probes used in real-time PCR

Target	Gene target	Forward primer	Reverse primer	Probe	Labels	Reference
Genus Brucella	bcsp31 gene	5’GCTCGGTTGCCAATATCAATGC3’	5’GGGTAAAGCGTCGCCAGAAG3’	5’AAATCTTCCACCTTGCCCTTGCCATCA3’	5’Fluorophore-6-FAM, 3’Quencher BHQ1	[42]
B. abortus	alkB gene	5’GCGGCTTTTCTATCACGGTATTC3’	5’CATGCGCTATGATCTGGTTACG3’	5’CGCTCATGCTCGCCAGACTTCAATG3’	5’HEX 3’BHQ1	[42]
B. melitensis	BMEI1162 gene	5’AACAAGCGGCACCCCTAAAA3’	5’CATGCGCTATGATCTGGTTACG3’	5’CAGGAGTGTTTCGGCTCAGAATAATCCACA3’	5’Texas Red 3’BHQ2	[42]