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. 2021 Oct 30;14:145. doi: 10.1186/s13048-021-00883-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Differential expression of Fshr-1 (upper panel) and Fshr-3 (lower panel) transcripts in sheep OSE cells after treating with FSH. Fshr-1 transcripts (dark blue bars) in vitro after 0, 3 and 15 h or without (light blue bars) FSH treatment (A-C). Evidently Fshr-1 was not affected by FSH treatment. Whereas Fshr-3 after FSH (red bars) showed upregulation compared to without FSH (pink bars) treatment. Expression of Fshr-3 was maximal after 3 h (please note a change in Y-axis scale to appreciate a difference between (D-F). The relative expression levels of each transcript from three different experiments are represented individually [24]. These results of FSH action on normal sheep OSE cells via Fshr-3 in vitro are similar to those reported for tumorigenic cell line ID8 and discussed above in Table 1 [19]