FIG. 6.

Ectopic induction of puc and dpp by dTAK1 demonstrated by X-Gal staining (A and B) and in situ hybridization for dpp antisense probe (C and D) of stage 14 embryos. (A) puc-lacZ/+; (B) en-GAL4/UAS-dTAK1; puc-lacZ/+; (C) wild type; (D) en-GALY/UAS-dTAK1. puc and dpp expression in the leading-edge cells is indicated by arrows (A and C, respectively). Ectopic expression of dTAK1, controlled by en-GAL4, ectopically induces both puc and dpp in the embryonic ectoderm with a striped pattern (B and D). (E) en-GAL4/UAS-lacZ. en-GAL4 expression pattern is shown. Anterior is to the left, and dorsal is up.