FIG. 8.
Lateral view of the cuticle phenotypes of dominant negative dTAK1-expressing embryos of the following genotypes: (A) +/pannierMD237GAL4, as a wild-type control; (B and C) UAS-dTAK1-K46R/+; UAS-dTAK1-K46R/pannierMD237GAL4. (A) Wild-type cuticle illustrating the regular spacing of the denticle belt on the ventral side and complete closure of the epidermis on the dorsal side. (B and C) Expression of dTAK1-K46R (two copies of transgene) during embryonic development by means of pnr-GAL4 (32) causes various defects. Defects in the anterior structure, typically loss of the mouth hooks (normal position of the mouth hooks is indicated with arrows in panels A and B), are seen in 37% of embryos (n = 591). Embryos of this type are frequently exhibit a small whole in the anterior and dorsal side of the cuticle (B). In the most extreme cases, the embryo is completely open dorsally (6%, n = 591) (C). Arrows indicate the edge of the dorsal hole of the cuticle.