TABLE 1.
Bacterial strains used in this study
| Species | No. of strains tested | No. of PCR-positive strains | Species according to PCR typing | Sourcea |
|---|---|---|---|---|
| C. jejuni | ||||
| Human isolates | 66 | 66 | 65 C. jejuni, 1 C. colib | UU, RSÖ, Telelab |
| Veterinary isolates | 7 | 7 | C. jejuni | NVI |
| SLV-167 | 1 | 1 | C. jejuni | NFA |
| SLV-267 | 1 | 1 | C. jejuni | NFA |
| SLV-264 | 1 | 1 | C. jejuni | NFA |
| CCUG 11284 | 1 | 1 | C. jejuni | CCUG |
| C. coli | ||||
| Human isolate | 1 | 1 | C. coli | Telelab |
| Veterinary isolates | 14 | 14 | 13 C. coli, 1 C. jejunib | NVI |
| SLV-271 | 1 | 1 | C. coli | NFA |
| CCUG 11283 | 1 | 1 | C. coli | CCUG |
| C. lari | ||||
| Veterinary isolates | 3 | 3 | C. lari | NVI |
| CCUG 23947 | 1 | 1 | C. lari | CCUG |
| From seagulls | 2 | 2 | C. lari | NVI |
| 4219/92 | 1 | 1 | C. lari | NIPH |
| 1557/93 | 1 | 1 | C. lari | NIPH |
| 1089/94 | 1 | 1 | C. lari | NIPH |
| 2138/94 | 1 | 1 | C. lari | NIPH |
| C. upsaliensis | ||||
| Human isolate | 1 | 1 | C. upsaliensis | Telelab |
| Veterinary isolates | 6 | 6 | C. upsaliensis | NVI |
| From dogs | 5 | 5 | C. upsaliensis | NVI |
| CCUG 14913 | 1 | 1 | C. upsaliensis | CCUG |
| 14913 | 1 | 1 | C. upsaliensis | NIPH |
| C. hyointestinalis | 2 | 0 | NVI | |
| C. fetus subsp. fetus | 2 | 0 | NVI | |
| C. fetus subsp. venerealis | 1 | 0 | NVI | |
| C. mucosalis | 2 | 1c | NVI | |
| Helicobacter pylori | 8 | 0 | UU | |
| Salmonella enteritidis SLV-397 | 1 | 0 | NFA | |
| Salmonella typhimurium SLV-248 | 1 | 0 | NFA | |
| Salmonella dublin SLV-242 | 1 | 0 | NVI | |
| Salmonella bovismorbificans SLV-443 | 1 | 0 | SDIIC | |
| Shigella flexneri 820/92 | 1 | 0 | SDIIC | |
| Shigella boydii 915/94 | 1 | 0 | SDIIC | |
| Shigella sonnei 637/94 | 1 | 0 | SDIIC | |
| Escherichia coli | ||||
| SLV-165 | 1 | 0 | NFA | |
| O157:H7 81186 | 1 | 0 | SDIIC | |
| O157:H7 ATCC 43889 | 1 | 0 | SDIIC | |
| O157:H7 ATCC 43890 | 1 | 0 | SDIIC | |
| Yersinia enterocolitica SLV-408 | 1 | 0 | NFA | |
| Vibrio cholerae CCUG 34649 | 1 | 0 | CCUG | |
| Vibrio parahaemolyticus CCUG 19113 | 1 | 0 | CCUG | |
| Vibrio vulnificus CCUG 13448 | 1 | 0 | CCUG | |
| Staphylococcus aureus SLV-350 | 1 | 0 | NFA | |
| Staphylococcus saprophyticus SLV-013 | 1 | 0 | NFA | |
| Enterobacter cloacae SLV-164 | 1 | 0 | NFA | |
| Enterococcus durans SLV-078 | 1 | 0 | NFA | |
| Bacillus cereus SLV-160 | 1 | 0 | NFA | |
| Klebsiella oxytoca SLV-089 | 1 | 0 | NFA | |
| Clostridium perfringens SLV-442 | 1 | 0 | NFA |
a
NFA, National Food Administration, Uppsala, Sweden; SDIIC, Swedish Institute for Infectious Disease Control, Stockholm, Sweden; NVI, National Veterinary Institute, Uppsala, Sweden; Telelab, A/S Telelab, Skien, Norway; UU, University of Uppsala, Uppsala, Sweden; RSÖ, Örebro Medical Center Hospital, Örebro, Sweden; CCUG, Culture Collection of the University of Göteborg, Göteborg, Sweden; NIPH, National Institute of Public Health, Oslo, Norway.
b
The disagreements between the biochemical and genetic typing are discussed in the text.
c
One strain of C. mucosalis gave a faint band of the correct size when using 1.5 mM MgCl2. This band disappeared when the MgCl2 concentration was lowered to 0.5 mM.