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. 2021 Oct 30;7:328. doi: 10.1038/s41420-021-00722-7

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© The Author(s) 2021, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Western blot detected the expression of FBXL10 in normal breast epithelial cell and different types of breast cancer cells. The relative expression of FBXL10 was normalized by GAPDH with gray level ratio analysis. B, C Scratch wound-healing assay evaluated the effect of FBXL10 overexpression or knockdown on the migration of MCF7 (B) and MDA-MB-231 (C) cells respectively. D, E Transwell assay assessed the effect of FBXL10 overexpression or knockdown on the migration and invasion of MCF7 (D) and MDA-MB-231 (E) cells respectively. The bar graph showed the number of migrating and invading cells for each category of cells with three independent replications. *P < 0.05; **P < 0.01; ***P < 0.001. F Western blot assay tested the EMT markers (E-cadherin, Cytokeratin 18, N-cadherin and Vimentin) in MCF7 cells with FBXL10 overexpression. G Western blot tested the effect of FBXL10 knockdown with siRNAs on the expression of EMT markers in BT474 cells.